Muratti 05/16/07 - Study 2 hypothesis

STUDY 2 – PET’S CHOICE - DUNCAN’S METHOD

Object:
To study the motivation of the hamsters to play Metazoa Ludens applied method of Duncan’s.

APPLICATION 1:

Subjects:
• 20 Phodopus roborovskii hamsters aged 1-2 years
• Viral antibody and parasite free
• Uniformity: seeds, water
• Cage in room with controlled lighting and optimal temperature and humidity

Method:
It is hard to know if the hamsters want and like to play Metazoa Ludens. However, we can use indirect methods to measure the strength of the preference of the hamsters towards the game. The motivation of the hamsters can be assessed by recording the number of times the hamsters automatically stay in the structure or go into the structureto play Metazoa Ludens.

Hypotheses:
H1: After 4 weeks, the mean number of times for the hamsters to choose to play Metazoa Ludens will increase, that is the motivation to play Metazoa L udens will increase.

Duration: 4 weeks

Procedures:
All the hamsters were placed in their individual cages separated from the Metazoa Ludens structure. For 2 hours a day, the cages would be linked to the structure. The first 1.5 hours was for the hamsters to explore the new environment in order to wear off their inquisitiveness which could affect the result.
In the next 0.5 hour, a whistle was blown to signal the start of the game. The number of times the hamsters to remain in the structure (or enter the structure via the tunnel should they be in the cage at the whistle blow) was recorded.

Result:
petschoice.jpg

Statistical Analysis:
Using Wilcoxon signed-rank test for paired data (n=20) to analyze the result, the mean number of times taken for the hamsters to choose to play the game was shown to increase over the study period (z=3.9199, ρ more than 1.000, Wilcoxon signed-rank test).

Conclusion:
After 4 weeks, the hamsters have strong preference to play Metazoa Ludens as the mean number of times they choose to play the game increases.

APPLICATION 2

Subjects:
Meena and J

Method:
To achieve an accurate result, the indirect parameter used should be closely linked to the real motivation of the hamsters. Hence, the number of times that the hamsters go into the structure in the presence of the signal, in this case is the whistle sound, will be used instead of the old method in application 1. The number of times that the hamsters go back into the cage during the game is also noted as another parameter for indirectly measuring the motivation to play the game of the hamsters.

Hypotheses:
H2: After 4 weeks, the mean number of times for the hamsters to go into the structure will increase and the number of times for the hamsters to go back into the cage will decrease, that is the motivation to play Metazoa L udens and the precise reaction of them to signal will increase.

Duration: 4 weeks

Procedures:
All the hamsters were placed in their individual cages separated from the Metazoa Ludens structure. They were allowed to explore the new environment in 10 to 15 minutes. The reason we reduce the length of exploring period is because it was observed that after 15 minutes, the hamsters became distracted and started to try to escape through the hole and the corners.
In next 30 minutes, the hamsters were guided to go into the long tunnel which linked the cage and the structure together. After the hamsters went inside, fix the second short tunnels which has one closed end to each of the two ends of the long tunnel.
DSCF7401.jpg

The number of times for the hamster to go into the structure per 10 times whistling was recorded. If the hamsters went into the structure without the presence of signal sound, that time would not be counted.
DSCF7408.jpg
Finally, the hamsters would play Metazoa Ludens game. The number of times that the hamsters went back into the cage during the game was noted.

Statistical Analysis:
Using Wilcoxon signed-rank test for paired data (n=20) to analyze the result.

Expected result:
study2expectedresult.jpg

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